gorgeous
发表于 2025-3-23 09:44:51
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整洁
发表于 2025-3-23 16:42:24
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Exuberance
发表于 2025-3-23 20:21:09
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天气
发表于 2025-3-23 23:03:59
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阐释
发表于 2025-3-24 03:21:39
https://doi.org/10.1007/978-3-642-99611-5rganisms. Known as the CRISPR-Cas system, Cas endonucleases such as Cas9 and Cas12a (also known as Cpf1) and guide RNA (gRNA) complexes recognize and cleave the target DNA, allowing for targeted gene manipulation. Along with the Cas protein engineering, gRNA engineering has broadened the application
薄荷醇
发表于 2025-3-24 09:16:57
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anniversary
发表于 2025-3-24 13:35:31
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惰性女人
发表于 2025-3-24 18:12:14
,Schichtgesteine sind Geschichtsbücher,s first subjected to digestion by CRISPR-Cas9 in vitro and then to whole genome sequencing, which results in unusual patterns of straight alignments at on-target and potential off-target sites. Analysis of these data with the Digenome-seq computer program allows for identification of the in vitro cl
使激动
发表于 2025-3-24 21:32:48
Die Entfaltung der Wirbeltiere,ion activator-like effector nucleases (TALENs) show great promises for research and therapeutic applications. One major concern is the off-target effects generated by these nucleases at unintended genomic sequences. In silico methods are usually used for off-target site prediction. However, based on
Liability
发表于 2025-3-25 01:34:53
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