lutein
发表于 2025-3-21 18:40:12
书目名称Brain Development影响因子(影响力)<br> http://impactfactor.cn/if/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development影响因子(影响力)学科排名<br> http://impactfactor.cn/ifr/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development网络公开度<br> http://impactfactor.cn/at/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development网络公开度学科排名<br> http://impactfactor.cn/atr/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development被引频次<br> http://impactfactor.cn/tc/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development被引频次学科排名<br> http://impactfactor.cn/tcr/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development年度引用<br> http://impactfactor.cn/ii/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development年度引用学科排名<br> http://impactfactor.cn/iir/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development读者反馈<br> http://impactfactor.cn/5y/?ISSN=BK0190147<br><br> <br><br>书目名称Brain Development读者反馈学科排名<br> http://impactfactor.cn/5yr/?ISSN=BK0190147<br><br> <br><br>
galley
发表于 2025-3-21 23:07:07
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厨师
发表于 2025-3-22 03:54:06
Computational Methods in Applied Sciencesthe fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene knockout.
FUSC
发表于 2025-3-22 08:13:11
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毛细血管
发表于 2025-3-22 09:57:38
CRISPR/Cas9 Genome Editing to Study Nervous System Development in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene knockout.
indoctrinate
发表于 2025-3-22 14:38:03
A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hyeps of cell fate specification to be followed at a single cell resolution. This protocol describes preparation of . embryos, classical in situ hybridization protocol coupled with nuclear staining, and a guide to identify gene expression in specific precursors of the developing brain at neural plate stages of development.
态度暖昧
发表于 2025-3-22 18:24:57
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多样
发表于 2025-3-23 00:34:48
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凝视
发表于 2025-3-23 02:25:25
Vladimir Leble,George N. Barakosrotocols for wholemount and flat preparations of . embryos, which allow a more detailed analysis of gene expression patterns in relation to the cellular context of the early brain (and facilitate the identification of individual brain neuroblasts) using conventional light microscopy.
MEET
发表于 2025-3-23 07:47:47
Zhengshun Cheng,Torgeir Moan,Zhen Gaoin multiple colors. This is followed by a detailed protocol as to how to prepare samples for imaging. Finally, we provide specifications to facilitate multichannel image acquisition using confocal microscopy.