Covenant 发表于 2025-3-21 18:31:31
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Characterization of Endothelial Internalization and Targeting of Antibody—Enzyme Conjugates in Cell es and in vivo. Both specific delivery to cells and proper subcellular addressing of enzyme cargoes are important parameters of targeting. This chapter describes methodologies for evaluating the binding and internalization of labeled conjugates directed to endothelial surface adhesion molecules in c擦掉 发表于 2025-3-22 01:43:05
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Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminaseloitation of the catalytic properties of the microbial transglutaminase from . species (MTGase), that is, acyl transfer between γ-carboxamide groups and various primary amines, new techniques for the enzymatic modification of proteins were developed. One example of bioconjugation is the biotinylatioSarcoma 发表于 2025-3-22 22:59:25
Fluorescent Sample Labeling for DNA Microarray Analysesrom total ribonucleic acid (RNA) samples. Two procedures are based on first-strand complementary DNA synthesis by reverse transcription. Label is introduced either by direct incorporation of fluorescently labeled nucleotides or indirectly by incorporation of aminoally-dUTP and subsequent coupling ofGORGE 发表于 2025-3-23 04:28:19
High-Density Labeling of DNA for Single Molecule Sequencingtarget into complementary flurophore-labeled DNA, and an exonuclease for the successive hydrolysis of the completely dye-labeled DNA. Recently, we found that the wild-type Klenow fragment of . DNA polymerase I is well-suited for the synthesis of DNA in a reaction set-up that contains exclusively speCRAFT 发表于 2025-3-23 07:03:39
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