女歌星
发表于 2025-3-28 14:44:10
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VEIL
发表于 2025-3-28 21:59:44
Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransfe C-terminal CaaX motif at the C-terminus of a protein substrate. The addition of only four amino acid residues minimizes the influence on the native protein structure. In addition, a variety of isoprenoid analogs containing different bioorthogonal functional groups, including azides, alkynes, and al
geometrician
发表于 2025-3-29 02:53:38
,Homogeneous Antibody–Drug Conjugates via Glycoengineering,ties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload. These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-sp
钻孔
发表于 2025-3-29 04:56:35
Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria,, a non-IgG binding scaffold, in a specific . strain to obtain high yields of protein carrying both a thiol and an azide group. We provide straightforward protocols for strain-promoted azide–alkyne cycloaddition (SPAAC) and thiol–maleimide conjugation, and furthermore compare these conjugation chemi
熄灭
发表于 2025-3-29 08:43:55
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Apoptosis
发表于 2025-3-29 14:49:39
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构成
发表于 2025-3-29 16:52:47
https://doi.org/10.1007/978-3-658-06581-2quence called Tub-tag. Here we describe the one-step Tub-tag protein modification protocol in detail and explain its utilization to generate fluorescently labeled proteins for advanced applications in imaging and diagnostics.
思想上升
发表于 2025-3-29 21:40:10
,Engineering Dual Variable Domains for the Generation of Site-Specific Antibody–Drug Conjugates,iable-domain (DVD) format. Here we provide protocols for the engineering, expression, and purification of the DVDs used for this strategy. We also provide a protocol for DVD–drug conjugation and describe methods for their biochemical characterization, including a catalytic assay to monitor conjugation efficiency.
anthropologist
发表于 2025-3-30 03:58:11
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人造
发表于 2025-3-30 07:02:49
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