Palate 发表于 2025-3-23 12:32:07
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Teil 4: Ergebnisse des Theorietransfers,stics, and more. Most nonspecific chemical conjugation methods ligate onto any of a number of sites on the antibody, leading to multiple conjugated species, many of which perturb antibody function. To solve these problems, we used CRISPR/Cas9-edited hybridomas to introduce a Sortase tag (LPXTG) and忘川河 发表于 2025-3-23 20:46:24
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https://doi.org/10.1007/978-3-658-31142-1ediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody–intein–chitin binding domain (CBD) fusion protein in . SHuffle. T7 cells, in the commonly used Luria–Bertani (LB) medium. The combination of these factors results in a high yieldwatertight, 发表于 2025-3-24 02:59:54
https://doi.org/10.1007/978-3-658-31142-1y, pharmacokinetics, efficacy, and safety as well as improve manufacturing consistency. The SMARTag. technology platform offers a practical and efficient chemoenzymatic solution for site-specific protein modifications. A bioorthogonal aldehyde handle is introduced through the oxidation of a cysteineconference 发表于 2025-3-24 08:15:50
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https://doi.org/10.1007/978-3-658-06581-2nzyme tubulin tyrosine ligase incorporates coumarin-amino acids to the terminal carboxylic acid of proteins containing a short peptidic recognition sequence called Tub-tag. Here we describe the one-step Tub-tag protein modification protocol in detail and explain its utilization to generate fluoresceCODE 发表于 2025-3-24 16:40:36
RaumFragen: Stadt – Region – Landschaftnstructs) largely due to facile library preparation and high throughput screening. Positron-emitting fluorine-18 (half-life = 109.7 min) has a unique set of properties that match well with the pharmacokinetics of smaller sized constructs. Several indirect fluorine-18 labeling approaches have been de消息灵通 发表于 2025-3-24 20:07:33
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