沙漠 发表于 2025-3-23 11:31:32

Primary Kidney Cells,ral reasons. First of all, kidney cells can be grown in vitro from the animal of choice. Thus the results of tissue-culture studies can be more closely correlated with animal studies. Second, new tissue-culture systems can be developed that more closely resemble the kidney cells in vivo than presently available established kidney cell lines.

躲债 发表于 2025-3-23 16:44:57

Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Ceproliferation and differentiation of early B-lymphocyte lineage cells. This method can be used to obtain heterogeneous populations of immature precursors of the B-cell lineage greatly enriched from other hematopoietic cell types. Clonal populations can also be obtained by extension of this method to limiting dilution culture.

Multiple 发表于 2025-3-23 19:20:32

1064-3745 The- fore, we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the m

丛林 发表于 2025-3-23 23:38:23

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Between 发表于 2025-3-24 03:37:54

https://doi.org/10.1007/978-3-476-05543-9ies. In humans, current theories are that B-lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T- and B-cells are most easily obtained either from peripheral blood or from biopsy of lymphoid tissues (lymph nodes, spleen, Peyer’s patches from gut, tonsils, and adenoids).

胰脏 发表于 2025-3-24 09:05:29

https://doi.org/10.1007/978-3-476-03711-4dy of skin epithelial stem cells because the epithelial cells of the outer root sheaths of the hair follicle are pluri-potential in their phenotype, and can re-epithelialize the denuded epidermis to heal wounds (.).

使声音降低 发表于 2025-3-24 14:27:03

Ideenkampf und »pacifìke Mission«eover, the stem cells from which muscle develops in tissue culture seem to be very resilient; they will survive anoxic conditions within muscle at temperatures between 4 and 37°C, for up to a few days, and when obtained in suspension, are easy to freeze (with 10% dimethyl sulfoxide as a cryoprotectant) and store in liquid nitrogen.

defendant 发表于 2025-3-24 17:43:11

https://doi.org/10.1007/978-3-476-03651-3ryonic stem cell lines is that they can be manipulated to differentiate into a diversity of cell types either directly in the tissue-culture dish or within the context of the normal developing embryo following their return to the embryonic environment.

格言 发表于 2025-3-24 21:40:26

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头脑冷静 发表于 2025-3-24 23:21:50

Hollow-Fiber Cell Culture,nd removes CO. and other waste products. This description applies equally to hollow-fiber culture systems but with culture medium in the place of blood, and with capillaries made from ultrafiltration or microfiltration membranes.
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查看完整版本: Titlebook: Basic Cell Culture Protocols; Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997