驱逐
发表于 2025-3-21 16:15:54
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Rustproof
发表于 2025-3-21 23:30:24
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Cupping
发表于 2025-3-22 02:03:48
Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging,ery low frequency. On the other hand, in vitro packaging limits the size of the DNA based on the phage head stability. However, in vitro package is the most efficient method for introducing large-insert clones.
crescendo
发表于 2025-3-22 04:49:50
BAC Finishing Strategies,PCI-13 from P. de Jong (.) were fingerprinted, and the resulting maps of these overlapping clones have been integrated with clone maps of individual chromosomes generated by other centers (.). Sets of minimal overlapping clones have been selected for sequencing from the resulting maps.
自制
发表于 2025-3-22 08:52:24
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Projection
发表于 2025-3-22 13:27:37
George J. Vachtsevanos,Kimon P. Valavanisse it relies on characterization of random clones in libraries comprising YACs or BACs. Even if a YAC or BAC contains an entire gene, the specific recovery of the gene was an arduous process. Often a gene is available as a contiguous set of fragments that must be pieced together.
Ebct207
发表于 2025-3-22 20:27:01
Sexual Minority Groups and Urban Healthe found that templates produced through RCA yield more consistent and higher-quality sequence than identical templates generated from plasmid-prep methods. The protocol is simple, amenable to high throughput, and currently in use at the DOE Joint Genome Institute (Walnut Creek, CA) for the daily production of 30,000 sequencing templates.
连累
发表于 2025-3-22 22:50:54
Tree Roots versus Sidewalks and Sewers,e the sequences contained in them. Thus, both physical and functional mapping objectives would be facilitated by an efficient strategy for making nested deletions from one end of the insert DNA in a BAC or PAC clone.
NEX
发表于 2025-3-23 02:59:39
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hidebound
发表于 2025-3-23 07:53:22
Rolling Circle Amplification for Sequencing Templates,e found that templates produced through RCA yield more consistent and higher-quality sequence than identical templates generated from plasmid-prep methods. The protocol is simple, amenable to high throughput, and currently in use at the DOE Joint Genome Institute (Walnut Creek, CA) for the daily production of 30,000 sequencing templates.