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发表于 2025-3-28 14:45:00
Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitroion often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.
改良
发表于 2025-3-28 20:08:56
In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regenerationos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.
隐语
发表于 2025-3-29 01:55:36
Axon Growth and Regeneration978-1-4939-0777-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
尖叫
发表于 2025-3-29 05:01:45
Milan Gopal,Tariq Abbas,J. L. Pippi Salleion often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.
Fissure
发表于 2025-3-29 10:18:51
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minimal
发表于 2025-3-29 12:12:42
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Lamina
发表于 2025-3-29 17:37:30
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oxidant
发表于 2025-3-29 23:14:26
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否认
发表于 2025-3-30 01:38:18
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延期
发表于 2025-3-30 06:36:08
1064-3745 ctions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and r978-1-4939-4584-9978-1-4939-0777-9Series ISSN 1064-3745 Series E-ISSN 1940-6029