冥想后
发表于 2025-3-25 06:37:52
Rational Design of Artificial ON-Riboswitches,hich is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.
百灵鸟
发表于 2025-3-25 11:03:52
Construction of Ligand-Responsive MicroRNAs that Operate Through Inhibition of Drosha Processing,, the engineered microRNAs can be readily modified to target different sequences and to bind different ligands. Individual miRNAs also can be incorporated into the same transcript for tunable, multi-gene silencing.
种子
发表于 2025-3-25 14:03:43
Plato’s , as an Ethical Performancers against hexa-histidine-tagged proteins. For the efficient enrichment of higher affinity aptamers, the selection stringency should be gradually increased. Undesired RNA species that bind to affinity resins can be eliminated from the pool by using a negative selection step and alternating different types of resins.
阴谋小团体
发表于 2025-3-25 16:50:35
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Vital-Signs
发表于 2025-3-25 22:03:55
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Robust
发表于 2025-3-26 02:22:10
Charlie Kuppens,Catherine M. Robby of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators.
callous
发表于 2025-3-26 04:19:08
Extrahuman Transcendence in Oscar Wilde’s quence with potential binding affinity from a pool of random sequences and designed a protocol for RNA 3D structure prediction. The proposed approach significantly reduces the RNA sequence search space, thus accelerating the experimental screening and selection of high-affinity aptamers.
农学
发表于 2025-3-26 11:45:45
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腐烂
发表于 2025-3-26 14:49:29
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magnanimity
发表于 2025-3-26 20:33:48
Dual-Selection for Evolution of In Vivo Functional Aptazymes as Riboswitch Parts,phenylalanine and a hammerhead ribozyme. Because this method uses selection, it does not rely on sequence-specific design and thus should be generalizable for the generation of in vivo operational aptazymes that respond to any targeted molecules.