neutralize 发表于 2025-3-25 04:13:35

Marcelo Fornazin,Luiz Antonio Joiags-CaM (rCML) in . and other Solanaceae plants, including potato (.). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.

破布 发表于 2025-3-25 08:03:47

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BUOY 发表于 2025-3-25 12:37:57

CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants,gs-CaM (rCML) in . and other Solanaceae plants, including potato (.). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.

Junction 发表于 2025-3-25 17:46:18

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incontinence 发表于 2025-3-25 23:01:07

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隐士 发表于 2025-3-26 02:58:28

Hong Joo Lee,Minsik Lee,Habin Leeolved have been genetically or biochemically identified in .. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzes . mutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the r

商议 发表于 2025-3-26 05:51:31

Hanne Höglund Rydén,Sara Hofmann,Guri Vernenisms has been extensively promoted by virus-induced gene silencing (VIGS) technique, which decreases the transcriptional level of the gene of interest via RNA silencing machinery triggered by the partial gene fragment inserted into a viral vector. This technique has contributed to addressing the fu

Gobble 发表于 2025-3-26 11:38:39

Ida Lindgren,Csaba Csáki,Anneke Zuiderwijk viruses often results in the breakdown of virus resistance in plants, which leads to a disastrous outcome in agricultural production. Although studies have analyzed the nature of plant virus resistance breakdown, it is still difficult to foresee the breakdown of a given virus resistance gene. In th

convert 发表于 2025-3-26 13:54:11

Ida Lindgren,Csaba Csáki,Anneke Zuiderwijknt tissues or cells. Thus, with a few exceptions, plant cell extracts are contaminated by vacuole-derived lytic enzymes and unsuitable for biochemical analyses. Here, we describe a method for removing the vacuoles from intact tobacco BY-2 protoplasts and for cell-free translation and replication of

舞蹈编排 发表于 2025-3-26 18:27:41

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查看完整版本: Titlebook: Antiviral Resistance in Plants; Methods and Protocol Kappei Kobayashi,Masamichi Nishiguchi Book 2019 Springer Science+Business Media, LLC,