杀虫剂 发表于 2025-3-26 22:54:52

Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differee transfection (.) and genetic disruption of genes in embryonic stem (ES) cell lines to produce transgenic animals (.,.). Currently, the most common cell line used is the mouse ES cell, but ES cells have recently been derived for other species (rabbit, rat, and hamster) (.–.).

Conspiracy 发表于 2025-3-27 01:44:00

Effect of Cis-Located Human Satellite DNA on Electroporation Efficiencyually random with respect to both the exogenous DNA and the particular human chromosome (.). Therefore, it can be expected that not only the expression, but also the stability and copy number of the transfected gene will depend on the integration sites and chromosomal flanking sequences.

Myelin 发表于 2025-3-27 06:44:16

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夸张 发表于 2025-3-27 09:29:05

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变异 发表于 2025-3-27 16:24:19

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魔鬼在游行 发表于 2025-3-27 20:07:30

Hans Dieter Klenk,Werner Slenczka cells (.–.). Electroporation has the advantage over other techniques in that many cells can be treated simultaneously and one or more compounds or macromolecules, such as proteins, can be introduced into cells at the same time (..).

Accord 发表于 2025-3-28 01:50:55

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GULF 发表于 2025-3-28 03:25:29

P. Flammang,R. Santos,D. Haesaertsually random with respect to both the exogenous DNA and the particular human chromosome (.). Therefore, it can be expected that not only the expression, but also the stability and copy number of the transfected gene will depend on the integration sites and chromosomal flanking sequences.

蒸发 发表于 2025-3-28 08:40:58

P. Flammang,R. Santos,D. Haesaertsir activity. Some established animal cell lines can be used as production hosts since they are easily transfected, can grow to high cell densities, and can be propagated in low-serum or serum-free medium.

严重伤害 发表于 2025-3-28 11:53:32

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查看完整版本: Titlebook: Animal Cell Electroporation and Electrofusion Protocols; Jac A. Nickoloff Book 1995 Springer Science+Business Media New York 1995