atopic-rhinitis 发表于 2025-3-27 00:38:52

https://doi.org/10.1007/978-94-017-6236-6 are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

Soliloquy 发表于 2025-3-27 05:11:16

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破布 发表于 2025-3-27 08:07:17

Sampling and Extraction of Ancient DNA from Sediments, extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

licence 发表于 2025-3-27 13:05:29

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thwart 发表于 2025-3-27 16:34:59

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植物茂盛 发表于 2025-3-27 21:41:41

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hematuria 发表于 2025-3-28 01:49:34

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溺爱 发表于 2025-3-28 03:33:46

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种植,培养 发表于 2025-3-28 09:58:25

Hiroyuki Yamazaki,Tomoaki Shirao working with ancient samples, including providing guidelines for setting up a new ancient DNA laboratory. We also discuss steps that can be taken at the sample collection and preparation stage to minimize the potential for contamination of ancient DNA experiments with exogenous sources of DNA.

女歌星 发表于 2025-3-28 11:54:51

Role of Drebrin in Synaptic Plasticityeach) pretreatment to destroy contaminant DNA that may be bound or otherwise attached to the surface of bone/tooth powder. The second, less destructive pretreatment uses a phosphate buffer to release surface-bound DNA.
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查看完整版本: Titlebook: Ancient DNA; Methods and Protocol Beth Shapiro,Axel Barlow,André E. R.‘Soares Book 2019Latest edition Springer Science+Business Media, LLC,