要求 发表于 2025-3-21 17:22:55
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Necropsy Procedures and Evaluation of Macroscopic Lesions of Pigs Infected with African Swine Fevergy, or virology as core basis of scientific research. Necropsies are an essential tool in veterinary pathology for disease investigation and should be conducted in a routine, systematic, and standard approach. An orderly necropsy procedure will allow the prosector (veterinary clinicians or veterinar成绩上升 发表于 2025-3-22 02:35:53
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Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses,further our understanding of the virus–host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFVsparse 发表于 2025-3-22 15:01:25
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Genotyping of African Swine Fever Virus,is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, foll渐强 发表于 2025-3-23 02:41:20
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Indirect Immunoperoxidase Test (IPT) for Detection of Antibodies Against African Swine Fever Virus hrough the action of the peroxidase enzyme. In this procedure, African green monkey established cell lines, such as Vero or MS, are infected with African swine fever virus (ASFV)-adapted isolates to these cell cultures. The infected cells are fixed and then used as antigens to determine the presence