detumescence 发表于 2025-3-21 18:00:44
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Experiences with Tritiated Compounds Prepared by Exposure to Tritium Gaspounds. Despite the absence of an adequate theory for predicting the specific activity attainable with a particular substance, the method has proven successful in labeling a great variety of compounds, including biologically active materials such as ribonucleic acid, lysozyme , and insulin . I灌输 发表于 2025-3-22 03:20:39
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Tritium Gas Exposure Labelingritium labeling. Experience has confirmed the initial report that activities in the range of 1–100 mc/g are generally obtained by exposure to curie amounts of tritium for several days, and that labeling is accompanied by the formation of tritiated by-products of high specific activity. Information oconference 发表于 2025-3-22 10:28:44
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Some Experiences with Labeling Nucleotides. This observation followed largely from the initial work of Leloir and his collaborators and of Park . Work by Cifonelli and Dorfman showed that Group A streptococci contain UDP-glucuronic acid (UDPGA), UDP-N-acetylglucosamine (UDPAG), and UDP-N-acetylmuramic acid. The first two, UDPGA aAPRON 发表于 2025-3-22 19:37:36
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Tritium Labeling by Other Methodsables one to obtain considerably higher specific activities and specifically labeled positions. Thus, the tritium gas exposure method of labeling should be considered only after other synthetic routes have been eliminated or if a randomly labeled molecule is required.可卡 发表于 2025-3-23 06:06:36
Preparation of Tritium-Labeled Paromomycin (Humatin) by Fermentation in a Medium Containing Tritiaten radiochemically pure paromomycin following exposure to tritium gas by the Wilzbach technique prompted us to try biosynthesis. While biosynthetic incorporation of hydrogen isotope from water into organic compounds has been reported frequently in the literature , this technique has not been