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https://doi.org/10.1007/978-3-030-63982-2t pulse width (usually picoseconds or femtoseconds) as well as repetition rates in the kilohertz to megahertz range. The repetition rate should ideally be tunable in order to adapt the pulse period to the required measurement window. In the blue, red, and IR spectral range, such pulses with energiesBRIDE 发表于 2025-3-22 23:01:55
https://doi.org/10.1007/978-3-030-63982-2al-focus FCS (2fFCS). We decided to put our focus on a detailed discussion of these two – and in our eyes well-merited – advanced methods, rather than giving an overview over the broad variety of advanced FCS methods that would consequently lack detail and leave the reader rather uneducated on all tcardiopulmonary 发表于 2025-3-23 02:24:29
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https://doi.org/10.1007/978-3-319-24987-2nt spectral properties. PIE was originally introduced to enable artifact-free fluorescence cross-correlation measurements, while first experiments with alternating laser excitation (ALEX) used the dual excitation of donor and acceptor for single-pair Förster resonance energy transfer (spFRET). In th