日月等 发表于 2025-3-21 17:20:20
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Die ertragsteuerliche Organschaftquestions. We explain technical aspects of sample preparation and image acquisition that will help in obtaining good diffraction-unlimited pictures. We also present embedding techniques adapted for ultrathin sectioning, which allow optimal 3D resolutions in virtually all biological preparations.Antigen 发表于 2025-3-22 01:57:13
https://doi.org/10.1007/978-3-642-51985-7commercial system. We explain how TIRF can be combined with other microscopy modalities and describe how to use TIRF to study processes such as endocytosis, exocytosis, and focal adhesion dynamics. Finally, we provide a step-by-step guide to imaging and analyzing focal adhesion dynamics in a migrating cell using TIRF microscopy.轨道 发表于 2025-3-22 06:19:14
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https://doi.org/10.1007/978-3-322-85310-3signaling events inside living cells, e.g., Protein–Protein interactions. In this chapter, we describe the calibration of both time-correlated single-photon counting (TCSPC) and frequency domain (FD) FLIM systems and the acquisition and analysis of FLIM-FRET data for investigating Protein–Protein interactions in living cells.Intersect 发表于 2025-3-22 23:37:51
https://doi.org/10.1007/978-3-322-85310-3ipt language. The simulation tool fits very well in a systems biology research setting that aims to maintain an interactive cycle of experiment-driven modelling and model-driven experimentation: the model and the experiment are in the same simulation. The full program can be obtained by request to the authors.冷峻 发表于 2025-3-23 02:42:14
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