jaunty
发表于 2025-3-23 11:18:49
http://reply.papertrans.cn/15/1456/145592/145592_11.png
顾客
发表于 2025-3-23 17:06:02
http://reply.papertrans.cn/15/1456/145592/145592_12.png
Amenable
发表于 2025-3-23 22:02:45
Die ertragsteuerliche Organschaftluorescent proteins (PA-FPs) as PALM probes. After reviewing the basics of 2D PALM, we detail astigmatic and multiphoton imaging approaches well suited to working with PA-FPs. We also discuss the use of open-source localization software appropriate for 3D PALM.
Constrain
发表于 2025-3-24 00:46:26
http://reply.papertrans.cn/15/1456/145592/145592_14.png
滔滔不绝地说
发表于 2025-3-24 03:40:35
https://doi.org/10.1007/978-3-662-26592-5ration, owing to the longer excitation wavelength required and to the ability to collect scattered emission photons as a useful signal. It also minimizes photodamage because lower energy photons are used and because fluorescence is confined to the geometrical focus of the laser spot. 2PE is therefor
orthopedist
发表于 2025-3-24 09:44:36
Kinder in einer globalisierten Welte protocol allows the recording of all phases of spheroid formation in three dimensions, including cell proliferation, aggregation, and compaction. We employ the human hepatic cell line HepaRG transfected with the fusion protein H2B-GFP, i.e., a fluorescing histone. The protocol allows monitoring th
venous-leak
发表于 2025-3-24 14:42:53
Ulrich Hagemann,Harald Seehausenomated preparation of “ready to transfect” multiwell plates and cell arrays, on which cells can be grown which are then reversely transfected with one type of siRNA in every individual well or spot. Additionally, different microscope types for screening approaches are compared and considerations abo
专横
发表于 2025-3-24 15:01:11
http://reply.papertrans.cn/15/1456/145592/145592_18.png
Endometrium
发表于 2025-3-24 19:25:25
https://doi.org/10.1007/978-3-322-85310-3s to advanced clinical diagnosis. Fluorescence lifetime imaging microscopy (FLIM) is now routinely used in the biological sciences to monitor dynamic signaling events inside living cells, e.g., Protein–Protein interactions. In this chapter, we describe the calibration of both time-correlated single-
Heterodoxy
发表于 2025-3-25 00:50:05
https://doi.org/10.1007/978-3-322-85310-3antitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language. The simulation tool fits very well in a systems biology research setting that aims to maintain an interactive cycle of experiment-driven