钢盔
发表于 2025-3-26 22:50:42
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CLAMP
发表于 2025-3-27 01:47:07
Methods in Molecular Medicinehttp://image.papertrans.cn/a/image/144887.jpg
不能妥协
发表于 2025-3-27 07:44:57
Franco H. Falcone,Bernhard F. GibbsHere we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.
无聊的人
发表于 2025-3-27 11:24:09
Franco H. Falcone,Bernhard F. Gibbsromoter(s) of interest. The latter parameter is assessed by quantification of viral DNA by hybridization of membrane-bound DNA following purification of DNA from nuclear fractions of adenovirus-infected cells. Two alternative protocols, primer extension and quantitative reverse transcription polymer
鲁莽
发表于 2025-3-27 14:16:40
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AUGUR
发表于 2025-3-27 19:53:52
Edward F. Knol,Bernhard F. Gibbsem. Here we present a collection of in vitro methods to study RNAi and the effect of an adenovirus infection on RNAi. We describe methods to measure the two key enzymatic complexes involved in RNAi: Dicer and RISC.
独轮车
发表于 2025-3-27 23:13:39
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GORGE
发表于 2025-3-28 03:13:10
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发微光
发表于 2025-3-28 09:38:58
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肿块
发表于 2025-3-28 13:49:32
Rick A. Adams,Scott C. Pedersenon between a monoclonal antibody and a protein of interest. Providing that the antibody—protein interaction does not interfere with the ability of the protein to interact with other proteins in a cell lysate, a protein complex containing the antibody, the protein of interest, and its interacting pro