Intimidate
发表于 2025-3-21 17:27:27
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Temporal-Lobe
发表于 2025-3-21 20:22:49
Methods in Molecular Biologyhttp://image.papertrans.cn/012/image/100701.jpg
Ige326
发表于 2025-3-22 03:07:44
https://doi.org/10.1007/978-1-4939-7021-6Hydrogels; 3D Organoids; Scaffolds; Organotypic cultures; Micropatterning; Microfluidics; Bioprinting; Thre
arboretum
发表于 2025-3-22 06:32:20
978-1-4939-8370-4Springer Science+Business Media LLC 2017
恶心
发表于 2025-3-22 12:09:50
http://reply.papertrans.cn/11/1008/100701/100701_5.png
ECG769
发表于 2025-3-22 15:29:30
https://doi.org/10.1007/978-3-322-83139-2–cell and cell–extracellular matrix interactions, 3D cell culture enables proper structural architecture and differentiated function of normal tissues or tumors in vitro. Thereby 3D cell culture realistically models in vivo tissue conditions and processes, and provides in vivo like responses. Since
Hallmark
发表于 2025-3-22 19:03:08
https://doi.org/10.1007/978-3-322-83139-2bserved in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM) proteins necessary for proper cell function. Enzymatic digestion of decel
hegemony
发表于 2025-3-22 23:02:20
https://doi.org/10.1007/978-3-322-83244-3 with native extracellular matrix (ECM) proteins, nonnative biopolymers, or synthetic polymers are often used to study these processes in vitro in 3D cell culture experiments. However, each of these materials systems present major limitations when used in mechanobiological studies. While native ECM-
Nonconformist
发表于 2025-3-23 01:32:39
https://doi.org/10.1007/978-3-322-83244-3of advanced three dimensional (3D) culture models. Herein, we report methods for the PEG-GAG gel-based 3D co-cultivation of human umbilical vein endothelial cells, mesenchymal stromal cells, and different cancer cell lines. The resulting constructs allow for the exploration of interactions between s
negligence
发表于 2025-3-23 07:30:52
https://doi.org/10.1007/978-3-322-83253-5sical cell reprogramming process. Surprisingly, the contribution of the three-dimensional (3D) microenvironment to iPSC generation has been largely overlooked. Here we describe a protocol for the generation of iPSCs in defined poly(ethylene glycol) (PEG)-based hydrogels that, besides allowing higher