alliance 发表于 2025-3-30 11:29:55
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Gene Targeting in the Mouse, variety of biological research areas. More recently, the original knock-out technology has been further refined to develop advanced strategies to generate conditional knock-out and knock-in mice. In this chapter, an overview of gene-targeting strategies is presented and procedures to generate these genetically engineered mice are discussed.NAVEN 发表于 2025-3-30 18:54:20
Manipulation of Gene Function in ,,mRNAs, respectively, into the early embryo. Herein we offer detailed protocols describing how to design and perform these experiments successfully, as well as a brief discussion of considerations for performing a microarray analysis in this organism.phlegm 发表于 2025-3-30 21:04:39
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, Genetics,ods for genetic analysis of ., including protocols for controlled crosses both by natural spawning and by the surgical isolation of gametes; the identification and propagation of mutant lines; and strategies for positional cloning.harbinger 发表于 2025-3-31 12:21:33
Reptile Embryology,stological techniques (whole-mount histochemistry and immunohistochemistry). In addition, we provide a summary of basic reptilian husbandry requirements and discuss important details of embryonic nutrition, egg anatomy and sex determination.Accessible 发表于 2025-3-31 14:14:06
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Manipulating and Imaging the Early , Embryo,cellular levels. The year-round availability of easily fertilized eggs, the embryo’s large size and rapid development, and the hardiness of both adults and offspring against a wide range of laboratory conditions provide unmatched advantages for a variety of approaches, particularly “cutting and past广告 发表于 2025-4-1 01:43:48
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