jet-lag
发表于 2025-3-25 05:13:56
Synthetic Gene Design Using Codon Optimization On-Line (COOL)y developed Codon Optimization On-Line (COOL) which is a web-based tool to provide multi-objective codon optimization functionality for synthetic gene design. COOL provides a simple and flexible interface for customizing codon optimization based on several design parameters such as individual codon
Tailor
发表于 2025-3-25 10:29:33
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平息
发表于 2025-3-25 13:49:55
Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size me, ligase, exonuclease, or recombinase in .. This technology can be applied to common . hosts (e.g., DH5α, JM109, TOP10, BL21(DE3)). The protocol includes three steps: (1) generate DNA insert and linear vector backbone by regular high-fidelity PCR, where these two DNA fragments contain 3′ and 5′ ov
集聚成团
发表于 2025-3-25 19:51:03
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HOWL
发表于 2025-3-25 23:23:09
BASIC: A Simple and Accurate Modular DNA Assembly Method assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts . The BASIC standard defines a single entry vector for all parts flanked by the same prefix and suffix sequences and its idempotent nature means that the ass
愚蠢人
发表于 2025-3-26 04:03:11
Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotidesnostics, nanotechnology, and gene therapy. They are usually chemically synthesized. Here we describe an enzymatic method which enables us to synthesize pure oligonucleotides which can be up to several hundred long bases.
deciduous
发表于 2025-3-26 05:29:52
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Fraudulent
发表于 2025-3-26 09:40:54
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rheumatism
发表于 2025-3-26 15:17:55
The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesisom multiple off-products. This amplification step can cause stochastic sampling effects that propagate errors during the synthesis and lower the variability when applied towards the construction of randomized libraries. We present the method for polymerase step reaction (PSR), a simple DNA polymeras
蔑视
发表于 2025-3-26 20:18:42
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