非决定性
发表于 2025-3-21 18:47:05
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我不明白
发表于 2025-3-21 22:34:19
Amine Coupling Through EDC/NHS: A Practical Approach,sis to optimize immobilization and regeneration. Topics in focus are different coupling techniques for small and large molecules, streptavidin–biotin sandwich immobilization, and optimizing regeneration conditions.
偏狂症
发表于 2025-3-22 00:34:27
High-Throughput Kinase Assay Based on Surface Plasmon Resonance,hosphorylation is calculated from the capture level of the substrates and the binding level of the anti-pTyr antibody monitored by SPR. A wide dynamic range and real-time monitoring of SPR contribute to improved data reliability, and optimization of the procedure for an array-based apparatus achieved multiple sample processing (1,000 samples/day).
ALE
发表于 2025-3-22 06:57:07
DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films,mit. We have shown a positive relation between the length of the used carbon spacer and the general performance of the DNA biosensor. In this chapter an extended protocol for the controlled immobilization and subsequent hybridization of DNA is described.
勋章
发表于 2025-3-22 12:30:57
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羊齿
发表于 2025-3-22 14:16:25
Affinity Constants for Small Molecules from SPR Competition Experiments,immobilization needed for each compound. An adaptation of the method to analyze interactions with bivalent analytes (e.g., antibodies) is also included. Some observed different affinities in solution compared to that on the SPR surface are discussed.
Creditee
发表于 2025-3-22 17:44:23
Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies,dings and need to be incorporated into a structure where it can reside in a natural environment. Supported liposomes offer such environment. In this chapter a new method is presented where multilayers of such supported liposomes are used to immobilize transmembrane proteins onto a solid sensor surface which is suitable for use in SPR detection.
阴郁
发表于 2025-3-23 00:20:38
Surface Plasmon Resonance: A General Introduction,omena. This introductory chapter deals with some specific features that are relevant to many diverse applications. The role and impact of kinetics in biomolecular interactions is highlighted. A concise description of the physical principles of the SPR phenomenon is given from a practical point of vi
EXALT
发表于 2025-3-23 02:00:27
The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterizationomolecular interactions between an immobilized and a soluble binding partner. The need to be aware of and critically test the assumptions underlying the analysis models is emphasized and the consequences for the experimental design are discussed. The two most common sources of deviation in SPR surfa
轻浮女
发表于 2025-3-23 09:28:21
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