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Albrecht Lüthke,Ingo Mülleroteins fused to a highly active bacterial alkaline phosphatase (PhoA) variant as reporter enzyme. Appropriate insertion of the DNA encoding the foreign peptides, proteic domains, or proteins between codons +6 and +7 of the . gene restores the initial frame of the . gene in the vector. Consequently,修饰语 发表于 2025-3-22 05:44:43
Albrecht Lüthke,Ingo Müllerng cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previouslNeutral-Spine 发表于 2025-3-22 09:19:48
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Albrecht Lüthke,Ingo Müller the pBRINTs-rAnb. family. These vectors offer the choice of using the antibiotics chloramphenicol, gentamycin, or kanamycin to select for chromosomal integration events. In addition, it is possible to eliminate these chromosomal antibiotic resistance markers, after integration has taken place. The条约 发表于 2025-3-22 19:26:40
Albrecht Lüthke,Ingo Müllerologous proteins in budding yeast (.) (.–.). This promoter is one of the most efficient yeast promoters and is used when a high level of constitutive gene expression is required. Most .-based vectors are constructed using high copy number plasmids, to maximize expression levels of heterologous sequedysphagia 发表于 2025-3-22 22:58:35
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Albrecht Lüthke,Ingo Müllerfermentation. However, optimizing conditions of the large-scale high-cell-density fermentation for biochemistry and industrialization is usually a laborious and time-consuming process. Furthermore, it is often difficult to produce authentic proteins in large quantities, which is a major obstacle foraggressor 发表于 2025-3-23 08:56:11
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