漠不关心
发表于 2025-3-21 20:00:22
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CRATE
发表于 2025-3-21 20:31:30
Jörg Zirener,Andreas HautkappeCas9 editing tool in model plants, the applications of this system have been increasingly reported in different plant species. This chapter reviews the contribution of the CRISPR/Cas9 system in the development of genetically modified crops with improved yield, nutritional value, and response to biot
声音刺耳
发表于 2025-3-22 02:22:53
ring with the critical step of transcription termination and 3′end formation provides a means for assaying the functional potential of a given RNA of interest..In this protocol, we describe a method for blocking 3′end processing of the nascent RNA transcript, by using RNase H-inactive antisense olig
Herbivorous
发表于 2025-3-22 05:24:31
Michael Tyroller,Daniela Nienstedt tag RNAs co-localized to the same particle. Sequencing such cDNA molecules identifies the RNA molecule and the barcode. Subsequent analysis of transcripts that share the same barcode, i.e., co-barcoding, reveals RNA co-localization and interactions. The technique is not restricted to pairs of RNAs
亲密
发表于 2025-3-22 11:46:45
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衍生
发表于 2025-3-22 14:32:24
Fredrik Metze,Lars Westpfahlng human cell extracts. We show that a half guide RNA (only one hairpin) is just as functionally competent as the full-length guide RNA (two hairpins) in guiding site-specific pseudouridylation in the human cell extracts. This discovery offers the opportunity for direct delivery of a short guide RNA
follicular-unit
发表于 2025-3-22 18:05:08
Rainer Riggertethod is to use short-wave ultraviolet (UV) light (254 nm), which causes direct crosslinking between the bases and adjacent proteins. However, this can result in large amounts of nonspecific damage to bases not actually involved in crosslinking; this limits, e.g., the ability to subsequently map the
致命
发表于 2025-3-23 01:06:57
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平静生活
发表于 2025-3-23 04:45:41
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跟随
发表于 2025-3-23 08:01:16
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