Collected
发表于 2025-3-28 17:16:33
Isolation and Accumulation of Spliceosomal Assembly Intermediatestag. Purification of arrested spliceosomes is carried out in two steps: (1) size exclusion chromatography and (2) affinity selection via a fusion of MS2 and maltose-binding protein (MBP). Complex assembly and purification are analyzed by denaturing polyacrylamide gel electrophoresis.
HATCH
发表于 2025-3-28 22:33:36
Regulation of Alternative Pre-mRNA Splicing-acting RNA-binding proteins. Here, we review the current state of understanding of the logic and mechanism of regulated alternative splicing and indicate how this understanding can be exploited to manipulate splicing for therapeutic purposes.
阐明
发表于 2025-3-29 00:25:26
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山间窄路
发表于 2025-3-29 03:29:15
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lanugo
发表于 2025-3-29 08:35:04
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Condense
发表于 2025-3-29 12:02:09
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狂热语言
发表于 2025-3-29 15:44:41
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有角
发表于 2025-3-29 22:19:23
Single Molecule Approaches for Studying Spliceosome Assembly and Catalysisflection fluorescence (TIRF) optics. Finally, we describe how to tether the pre-mRNA onto the passivated slide surface and introduce the SNAP-tagged cell lysate for analysis of spliceosome assembly by single molecule fluorescence.
大气层
发表于 2025-3-30 01:46:14
Alternative Pre-mRNA Splicingprotein function and ultimately impact biological outcomes. We examine the possibility that portions of alternatively spliced transcripts are the result of stochastic processes rather than regulated. We discuss the implications of misregulated alternative splicing and explore of the role of alternative splicing in human disease.
遗忘
发表于 2025-3-30 04:14:56
In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extracte tools and techniques necessary to carry out an in vitro splicing assay. Through the use of radiolabeled pre-mRNA and crude nuclear extract, spliced mRNAs can be purified and visualized by autoradiography for downstream analysis.