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Nicholas P. Pollis,Carol A. Pollisiding known pitfalls.. .Practical and adaptable, .Mammalian Cell Viability: Methods and Protocols. serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incre978-1-4939-6153-5978-1-61779-108-6Series ISSN 1064-3745 Series E-ISSN 1940-6029heterodox 发表于 2025-3-25 10:41:15
iding known pitfalls.. .Practical and adaptable, .Mammalian Cell Viability: Methods and Protocols. serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incre978-1-4939-6153-5978-1-61779-108-6Series ISSN 1064-3745 Series E-ISSN 1940-6029stress-test 发表于 2025-3-25 12:41:01
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Donald Granbergalian cells in targeted manners. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooti无政府主义者 发表于 2025-3-25 23:20:03
Robert R. Blake,Jane Srygley MoutonNA operator sequence CarO. The switch is characterized by high reversibility, high transgene expression levels, and low leakiness, leading to up to 350-fold induction ratios in mammalian cells. In this chapter, we describe the essential steps to build functional components of the green light-regulatDawdle 发表于 2025-3-26 01:23:16
Harry C. Triandissed SLIPT system for inducing (1) reversible protein translocation and (2) synthetic activation of the Raf/ERK pathway. This system provides a simple and versatile tool in mammalian synthetic biology for temporally manipulating various signaling molecules and pathways at the PM.craving 发表于 2025-3-26 05:55:57
Robert L. Montgomerytiplexed fashion. While many genome engineering technologies exist and provide a means to efficiently manipulate one or a few genes in a singular context-knockout, inhibition, or activation in a constitutive, conditional, or inducible manner-progress towards engineering complex cellular programs has消瘦 发表于 2025-3-26 09:02:39
ation advice from the experts.This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book掺和 发表于 2025-3-26 16:06:19
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