人工制品
发表于 2025-3-30 10:19:47
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ASSAY
发表于 2025-3-30 15:18:43
The Satellite Cell Colony Forming Cell Assay as a Tool to Measure Self-Renewal and Differentiation P of colonies derived from single satellite cells. The variables of cell survival (cloning efficiency), proliferative potential (nuclei per colony), and differentiation propensity (ratio of nuclei within myosin heavy chain-positive cytoplasm to total nuclei) can thus be obtained.
飞来飞去真休
发表于 2025-3-30 20:18:17
Co-cultures of Macrophages with Muscle Stem Cells with Fibroadipogenic Precursor Cells from Regeneraol allowing the isolation of primary muscle stem cells, macrophages, and fibroadipogenic precursors by Fluorescence Activated Cell Sorting (FACS) or Magnetic Cell Separation (MACS), together with co-culture methods using a specific setup for a short time window to keep as much as possible the . properties of the isolated cells.
现晕光
发表于 2025-3-30 21:50:55
High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubesning stem cell dynamics during regeneration and tissue maintenance. Here we described a method to assess mitochondrial respiration (oxygen consumption rate) and glycolysis (ECAR) in primary murine satellite cells, multinucleated myotubes, and C2C12 myoblasts.
florid
发表于 2025-3-31 00:55:46
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Nutrient
发表于 2025-3-31 06:41:26
Techniques for Injury, Cell Transplantation, and Histological Analysis in Skeletal Musclen (H&E) staining or antibodies are the simplest and standard methodologies for assessing skeletal muscle phenotypes. In this chapter, we describe fundamental techniques and protocols for inducing skeletal muscle regeneration by using chemicals and cell transplantation, in addition to methods of preparing and evaluating skeletal muscle samples.
ethereal
发表于 2025-3-31 12:40:10
Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloribe the appropriate procedures for tenotomy-induced mechanical overloading and tail-suspension-induced mechanical unloading, which are the most common and simple methods to induce muscle hypertrophy and atrophy in mouse models.
Foolproof
发表于 2025-3-31 15:45:29
Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Techniquere in mice and rats, as these nerves can be resected without great difficulty. An increasing number of reports have recently been published on experiments using a tibial nerve transection technique in mice. In this chapter, we demonstrate and explain the procedures used to transect the sciatic and tibial nerves in mice.