易碎
发表于 2025-3-23 10:36:40
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障碍
发表于 2025-3-23 17:00:41
Single Molecule FRET Analysis of DNA Binding Proteinsgle-molecule Förster Resonance Energy Transfer (smFRET) to probe the binding dynamics of individual proteins on single DNA molecules. We provide a detailed discussion of total internal reflection fluorescence (TIRF) instrument design, nucleic acid labeling with fluorophores, flow cell surface passivation, and data analysis methods.
anthropologist
发表于 2025-3-23 20:12:45
Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Sint, permitting simultaneous single-molecule visualization and mechanical manipulation. We provide a simple method to calibrate the evanescent wave penetration depth via supercoiling of DNA with a fluorescent nanodiamond-labeled magnetic bead and a complementary method employing a surface-immobilized fluorescent nanodiamond.
呼吸
发表于 2025-3-24 00:06:24
Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions on the trapped objects can be accurately measured and exerted. Optical tweezers can typically obtain a nanometer spatial resolution, a picoNewton force resolution, and a millisecond time resolution, which makes them excellently suited to study biological processes from the single-cell down to the s
Cabinet
发表于 2025-3-24 02:53:14
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Confidential
发表于 2025-3-24 09:42:53
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Fantasy
发表于 2025-3-24 14:41:52
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喃喃而言
发表于 2025-3-24 17:44:55
A Brief Introduction to Single-Molecule Fluorescence Methodsfollowing section of this volume. Fluorescence methods provide the sensitivity required to study biology on the single-molecule level, but they also allow access to useful measurable parameters on time and length scales relevant for the biomolecular world. Before several detailed experimental approa
恩惠
发表于 2025-3-24 21:12:18
Fluorescent Labeling of Proteinshe movement of proteins in order to gain insight into their biological functions. A prerequisite to these experimental approaches is to graft one or more fluorophores on the protein of interest with the desired photophysical properties. Here, we describe procedures for efficient methods used to cova
分期付款
发表于 2025-3-25 02:40:34
Single-Molecule Imaging of , Transmembrane Proteinse-molecule imaging due to their small size, only slightly larger than the diffraction limit of visible light. Here, we describe how reliable and reproducible single-molecule data can be obtained for a transmembrane protein in the Gram-negative bacterium . by using live-cell fluorescence microscopy.