giggle 发表于 2025-3-25 03:30:22
Book 2018 reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and practical, .Selenoproteins: Methods and Protocols .aims to ensure successful results in the further study of this vital field..Contort 发表于 2025-3-25 11:26:52
Book 2018ccessful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and practiSarcoma 发表于 2025-3-25 12:53:40
Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs(RBPs) and leaving behind proteins that are bound to the beads (Michlewski and Caceres, RNA 16(8):1673–1678, 2010). This chapter will describe the isolation of proteins that bind specifically to the distal region of the Selenoprotein S 3′ untranslated region (3′ UTR).简略 发表于 2025-3-25 19:22:35
Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profilingodons are redefined to encode selenocysteine. Here, we describe a streamlined protocol that was developed in our lab to process mammalian tissue to produce the requisite matched ribosome profiling and RNA-Seq libraries for deep sequencing.Frequency-Range 发表于 2025-3-25 23:29:05
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SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteinsnent of Seblastian, a pipeline for the identification of selenoprotein genes that employs SECIS finding as the first step. This chapter constitutes a practical guide to use SECISearch3 and Seblastian, which can be run via webservers at ./ or ..严厉谴责 发表于 2025-3-26 08:15:02
Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteinsnotate selenoprotein genes in genomic sequences. This program uses a SECIS-independent approach, based on homology searches, and employs curated built-in profile alignments for all known selenoprotein families. Selenoprofiles constitutes the most accurate method for predicting selenoprotein genes belonging to known families.RACE 发表于 2025-3-26 10:58:17
Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selaused by the binding. Here, we describe specific chemical approaches including chemical probing and site-directed or bifunctional reagent-mediated cross-linking, which have been used for exploring the mechanism of selenocysteine insertion into a polypeptide chain by mammalian ribosomes.Volatile-Oils 发表于 2025-3-26 15:16:30
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