漏出 发表于 2025-3-21 19:34:36
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A Method to Introduce an Internal Tag Sequence into a , Chromosomal Gene,information regarding its localization, its expression or its biological function. In this chapter, we describe a process, derived from the Datsenko and Wanner procedure, which allows the introduction of an internal 2HA tag sequence into a chromosomal gene of the bacterial pathogen ..打算 发表于 2025-3-22 03:03:18
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Quantitative Proteomic Identification of Host Factors Involved in the , Infection Cycle,e intracellular interplay with pathogens. This method allows identification of proteins subject to degradation or upregulation in response to intracellular infection. It can also be used to study intracellular dynamics (trafficking) of proteins in response to the infection. Here, we describe the ana滔滔不绝地讲 发表于 2025-3-22 20:00:57
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,Generation and Use of Site-Directed Chromosomal ,′ Translational Fusions in ,,ype III secretion system effectors from gram-negative pathogens like .. These fusions are usually generated using plasmids with strong promoters. Here, we describe a protocol to insert the CyaA′-encoding sequence in a specific site in the bacterial chromosome in order to get a monocopy fusion whose