坦白 发表于 2025-3-25 04:08:51
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Husbandry of Spanish Ribbed Newts (,)pter, we present detailed husbandry protocols for the Spanish ribbed newt (. ). We describe the main phases of their life cycle, with emphasis on the progressive development of sensory, motor, and integration systems, which lead to the acquisition of specific stereotyped (and conditioned) behaviors.宽宏大量 发表于 2025-3-25 19:11:37
Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studiesconducted in species belonging to the Plethodontidae family, where some of the species differs from other salamander families due to their direct development, thus absence of a larval life. Here, we describe a protocol to maintain the plethodontid salamanders of genus . species under laboratory condAscribe 发表于 2025-3-25 23:16:43
Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISHifferentiate and redifferentiate into lens cells. Recent studies have shown that nuclear architecture of PECs is dynamically changed and unique epigenetic regulation in somatic nucleus is crucial in the lens transdifferentiation. Immuno-FISH, detection of protein and gene loci in nucleus, is one of愉快么 发表于 2025-3-26 01:12:36
Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablations. Neuronal regeneration occurs in a subset of species, including lizards, teleost fish, axolotls, and newts. One tool for studying neuronal regeneration in the adult brain is intraventricular injection of selective neuronal toxins, which leads to loss of subpopulations of neurons. To trace cells in轻而薄 发表于 2025-3-26 07:44:22
The Accessory Limb Model: An Alternative Experimental System of Limb Regenerationy pathways and test for signaling molecules that regulate limb regeneration. Here, we summarize the history of the ALM and describe the specific details involved in inducing ectopic blastemas and limbs from a skin wound on the side of the arm.寒冷 发表于 2025-3-26 08:58:38
High-Efficiency Electroporation of the Spinal Cord in Larval Axolotlil. Particularly fascinating is their ability to regenerate a fully functional spinal cord after losing the tail. Electroporation of DNA plasmids or morpholinos is a valuable tool to gain mechanistic insight into the cellular and molecular basis of regeneration. It provides among other advantages a保全 发表于 2025-3-26 15:58:42
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