彩色
发表于 2025-3-28 15:24:01
Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice adopted as two of the most powerful genome editing systems in plants. Recently, we developed a single transcript unit (STU) CRISPR 2.0 toolbox for applications in plants, which contains two STU-Cas9 systems and one STU-Cas12a system. Here, we describe a detailed protocol about using the STU CRISPR
作呕
发表于 2025-3-28 22:01:53
Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9e effector nuclease (TALEN), such as multiplex genome editing. With multiplex genome editing, CRISPR-Cas9 becomes a preferred SSN to edit many quantitative trait loci (QTL) simultaneously for trait improvement in major crops. A multiplexed CRISPR system is also important for deletion of a large frag
太空
发表于 2025-3-29 02:34:40
FLP-Mediated Site-Specific Gene Integration in Rice is by using the yeast FLP-. recombination system that is efficient in directing DNA integration into the “engineered” genomic sites. The critical parameters of this method include the use of the thermostable version of FLP protein and the promoter trap design to select site-specific integration clo
尖酸一点
发表于 2025-3-29 06:07:21
http://reply.papertrans.cn/84/8302/830194/830194_44.png
inclusive
发表于 2025-3-29 07:49:16
Morphogenic Regulators and Their Application in Improving Plant Transformation elite germplasm of major crop species. This chapter will briefly review key regulatory genes involved in plant morphogenesis with a focus on in vitro somatic embryogenesis and their application in improving plant transformation.
阻碍
发表于 2025-3-29 12:32:30
http://reply.papertrans.cn/84/8302/830194/830194_46.png
Synapse
发表于 2025-3-29 16:05:09
http://reply.papertrans.cn/84/8302/830194/830194_47.png
孤僻
发表于 2025-3-29 20:46:46
http://reply.papertrans.cn/84/8302/830194/830194_48.png
Nomadic
发表于 2025-3-30 03:41:03
http://reply.papertrans.cn/84/8302/830194/830194_49.png
胆小懦夫
发表于 2025-3-30 04:24:52
http://reply.papertrans.cn/84/8302/830194/830194_50.png