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Tissue-Specific Ribosome Profiling in apable of profiling translation in specific tissues and cells are crucial. Here, we describe a method for profiling genome-wide translation in specific tissues or cell types in ., in which we combine ribosome affinity purification with ribosome profiling to enable a simplified protocol for robust an占线 发表于 2025-3-24 00:13:49
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Assessing Ribosome Distribution Along Transcripts with Polarity Scores and Regression Slope Estimateeferences. For comparison versus a control sample, . estimates an improved metric, the relative linear regression slope of coverage along transcript length. This involves de-noising by profile segmentation with a Poisson model and aggregation of Ribo-Seq coverage within segments, thus achieving reli人类的发源 发表于 2025-3-24 07:17:59
1064-3745 d practical, .Ribosome Profiling: Methods and Protocols. aims to provide the type of standardized protocols that have previously been unavailable in an effort to bypass the major barriers to wide use of ribosome profiling-based approaches..978-1-0716-1152-4978-1-0716-1150-0Series ISSN 1064-3745 Series E-ISSN 1940-6029追逐 发表于 2025-3-24 12:25:25
1064-3745expertsThis book provides a single-source reference on the current state of the ribosome profiling method by describing experimental protocols for the quantitative analysis of translation in a variety of model organisms. In addition, the volume presents a detailed overview of the existing softwareinsert 发表于 2025-3-24 16:47:48
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Identification of Translation Start Sites in Bacterial Genomes reading frames and reveals the presence of internal start sites within genes. Here, we provide a detailed Ribo-RET protocol for .. Ribo-RET can be adapted for mapping the start codons of the protein-coding sequences in a variety of bacterial species.Landlocked 发表于 2025-3-25 01:56:39
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