委屈
发表于 2025-3-25 03:29:16
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命令变成大炮
发表于 2025-3-25 09:51:35
Preparation and functional characterization of a catalytically active fragment of phosphorylase kina catalytic activity — with varying degrees of calcium dependence — result upon the digestion of phosphorylase kinase with assorted proteases. However, especially high yields of the chymotryptic fragment are obtainable, with purification on an Ultrogel-34 column and a DEAE Sepharose CL-6B column giving 23% of the maximum possible protein.
otic-capsule
发表于 2025-3-25 14:53:32
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faultfinder
发表于 2025-3-25 16:45:38
Networking with mitogen-activated protein kinasesmbine to assemble distinct modules for intracellular signal transmission. However, the fundamental architecture of these protein kinase cascades has been highly conserved during eukaryotic evolution. (Mol Cell Biochem . 157–169, 1993)
chemoprevention
发表于 2025-3-25 23:52:16
Expression, purification, characterization, and deletion mutations of phosphorylase kinase γ subunitloenzyme. All recombinant γs exhibit similar .. values for both substrates, i.e., about 18μM for phosphorylase . and about 75 μM for MgATP. Three truncated γs, γ., γ., and γ., have a 1.9- to 2.5-fold greater catalytic efficiency (../..) than that of the full-length wild-type γ and a 3.5- to 4.5-fold
Hemoptysis
发表于 2025-3-26 03:06:14
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追踪
发表于 2025-3-26 07:57:47
Expression of cGMP-dependent protein kinase in ,d to the aminoterminus of the cGMP kinase. (II) A gram./gram. shuttle vector for expression under the control of the tac promotor was used. Both constructs directed the synthesis of an insoluble and inactive cGMP kinase. These results suggest that large amounts of cGMP kinase can be expressed in .,
chuckle
发表于 2025-3-26 11:34:54
Chicken smooth muscle myosin light chain kinase is acetylated on its NH2-terminal methionine acid analysis and Edman sequencing of a CNBr-subfragment of the thermolytic peptide indicated that it had the composition and sequence, (Met)-Asp-Phe-Arg-Ala-Asn, with a calculated mass of 753. The difference in mass corresponds to the NH.-terminal Met being blocked by acetylation. The results demo
最初
发表于 2025-3-26 13:12:37
Serine phosphorylation of protein tyrosine phosphatase (PTP1B) in HeLa cells in response to analoguen the PTP from control and stimulated cells. Either multiple kinases phosphorylate a common site in the PTP1B, or a single kinase is activated ‘downstream’ of cAMP- and Ca./phospholipid-dependent kinases. The results indicate that phosphorylation of a serine residue in the segment 283–364, probably
食品室
发表于 2025-3-26 20:30:28
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