Callus
发表于 2025-3-23 11:54:07
, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.978-3-642-97481-6
keloid
发表于 2025-3-23 15:07:10
Book 19931st editionive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section sing
遍及
发表于 2025-3-23 20:16:10
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没有希望
发表于 2025-3-24 01:33:05
The Resinsuert et al, 1956; Glauert and Glauert, 1958; Luft, 1961; Glauert, 1965, 1975). The epoxy resins gave uniform curing, and were found to be beam-stable. Uncrosslinked methacrylate is only two-dimensionally bonded and is rapidly destroyed in the electron beam. It is also prone to shrinkage, sometimes u
Tailor
发表于 2025-3-24 02:20:58
Cryotechniques sensitive to even low concentrations of fixative, or for preserving structures that cannot be stabilised during fixation or dehydration (Sect. 1.3.2). The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can then be in to resin
avenge
发表于 2025-3-24 09:51:57
Methods for Resin Polymerisation Epoxides can only be cured by heat to produce sectionable blocks. It is possible but impracticable to cure Epoxy resins with UV-light or chemical catalysts (Glauert, 1975). However, considerably longer times are needed for the tissue to be present in an organic monomer (Sect. 1.3.1). These methods
Biomarker
发表于 2025-3-24 13:24:44
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Outwit
发表于 2025-3-24 16:00:20
Strategies in Immunolabelling tissue substances with fluorescent antibodies. Since then numerous ingenious markers have been attached to antisera and other macromolecules in order to identify tissue and cellular substances both in the light and the electron microscope. Ferritin (Singer, 1959) was the first EM marker, followed b
Surgeon
发表于 2025-3-24 19:36:16
General Considerationsng, collectively called the section pretreatment, and the staining procedures themselves. The requirement for section pretreatment depends on the extent of fixation and the type of resin, with epoxy resin sections of post-osmicated tissue needing the most attention, and acrylic resin sections of ald
Trypsin
发表于 2025-3-25 01:48:03
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