bronchodilator
发表于 2025-3-26 21:04:05
,,-Acting Ribozymes for the Production of RNA In Vitro Transcripts with Defined 5′ and 3′ Ends, at both the 5′- and 3′-ends. This chapter describes the use of .-acting ribozymes, 5′-end hammerhead (HH) and 3′-end hepatitis delta virus (HDV), for direct transcriptional processing to yield target RNAs with precisely defined ends. The method is focused on the use of the pRZ and p2RZ plasmids tha
phase-2-enzyme
发表于 2025-3-27 03:41:11
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使尴尬
发表于 2025-3-27 08:22:50
Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography and DNAzyme Methods,ription method results in heterogeneity at the RNA 3′-terminus. RNA purification requires single-nucleotide resolution to separate the transcript of the correct length from the aborted or add-on transcripts that are usually present in comparable amounts. Here, we describe an RNA preparation method t
Predigest
发表于 2025-3-27 13:22:50
,Preparation of λN-GST Fusion Protein for Affinity Immobilization of RNA, Affinity purification is particularly advantageous because it can be performed in a few hours under non-denaturing conditions. However, the performance of affinity purification methods can vary tremendously depending on the RNA immobilization matrix. It was previously shown that RNA immobilization
受人支配
发表于 2025-3-27 17:20:53
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dissent
发表于 2025-3-27 20:25:53
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Engulf
发表于 2025-3-28 00:12:18
In Vitro Transcription of Modified RNAs,o standard transcription protocols. RNAs containing a single phosphate or a cap structure at their 5′ ends can readily be generated either co-transcriptionally or through enzymatic treatments of transcription products. Likewise, a variety of modified bases, including fluorescent or biotinylated spec
medium
发表于 2025-3-28 05:21:11
End-Labeling Oligonucleotides with Chemical Tags After Synthesis,le nucleic acid detection, yield information about its state, and can serve as a handle by which the nucleic acid and associated factors can be purified from a mixture. Radioactive phosphate is commonly added to the 5′ or 3′ end of an oligonucleotide post synthesis using enzyme-catalyzed reactions.
abduction
发表于 2025-3-28 08:11:05
High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA,ndent on the presence of modified nucleotides in tRNA, each of which performs a distinct function. To better understand how individual modifications modulate tRNA function, a method to isolate and purify a site-specifically modified tRNA is essential. This chapter describes an enzymatic method to sy
懒鬼才会衰弱
发表于 2025-3-28 10:28:10
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