Monolithic
发表于 2025-3-26 22:25:59
Thomas Gassler,Lina Heistinger,Diethard Mattanovich,Brigitte Gasser,Roland Prielhofer
玷污
发表于 2025-3-27 05:10:26
Mari A. Piirainen,Heidi Salminen,Alexander D. Frey
keloid
发表于 2025-3-27 06:30:25
Anita Emmerstorfer-Augustin,Tamara Wriessnegger,Melanie Hirz,Guenther Zellnig,Harald Pichler
健谈
发表于 2025-3-27 13:16:53
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VOC
发表于 2025-3-27 15:12:55
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认为
发表于 2025-3-27 20:45:13
Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in ,itations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins. Here, . genetic tools and host strains, developed for authentic processing and modification of secretory recombinant proteins, are introduced with the analytical protocols.
Suppository
发表于 2025-3-28 01:51:24
Book 2019bleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Recombinant Protein Production in Yeast: Methods and Protocols. aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications. .
面包屑
发表于 2025-3-28 03:52:28
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enfeeble
发表于 2025-3-28 06:14:43
Selection of Heterologous Protein-Producing Strains in ,Here, we present the Golden Gate cloning strategy for the construction of multiple expression cassettes, the transformation into ., and the selection of efficient enzyme-producing strains using an insect alpha-amylase as a reporter detected via a thermal cycler-based microassay.
和谐
发表于 2025-3-28 14:18:55
Assessing the Quality of Recombinant Products Made in Yeast,determination of posttranslational modifications such as glycan analysis are not described. In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.