Jacket 发表于 2025-3-23 12:37:11

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在前面 发表于 2025-3-23 15:16:55

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不满分子 发表于 2025-3-23 19:13:21

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场所 发表于 2025-3-23 23:19:04

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拍翅 发表于 2025-3-24 02:27:15

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FACT 发表于 2025-3-24 07:13:10

CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors,roteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of a

古老 发表于 2025-3-24 11:00:09

Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHvariety of different scales ranging from bench to production scales, with working volumes from 10 mL to 25,000 L. Bench-scale STRs are commonly used to culture mammalian cells for process development, to troubleshoot production scale bioreactors using scale-down models (SDM), or to conduct fundament

同义联想法 发表于 2025-3-24 16:17:37

Continuous and Integrated Expression and Purification of Recombinant Antibodies,a perfusion bioreactor is discussed containing the preparation procedures, the seeding train and the preparation and control of a long-term production run. The downstream processes exploit the benefits of countercurrent chromatography. Their design from batch experiments is presented. The CaptureSMB

积云 发表于 2025-3-24 20:50:17

High Throughput Transfection of HEK293 Cells for Transient Protein Production,drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection reagent. An automated liquid handler can be used to perform pipetting steps for transfecting batches

滴注 发表于 2025-3-25 01:39:36

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查看完整版本: Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2018 Springer Science+Business Media, LLC, par