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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/823545.jpg木讷 发表于 2025-3-25 11:04:19
https://doi.org/10.1385/1592599273PCR; cell; enzymes; hybridization; ion channels; lipide; microscopy; protein; radiography; researchindoctrinate 发表于 2025-3-25 15:30:43
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,Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR,oximately one-half of all drugs currently marketed exert their actions, either directly or indirectly, via GPCRs (.). Given the potential commercial opportunities emanating from the identification of small molecule modulators of “novel” GPCRs (currently, GPCRs generate in excess of $25 billion per ymusicologist 发表于 2025-3-26 08:45:08
mRNA,hern hybridization, first described by Alwine et al. (.), has become a very powerful technique in many research areas, including that of receptor research. The applications of these techniques are many and include (1) direct assessment of the presence, distribution, and modulation under different ph阻挠 发表于 2025-3-26 14:25:59
Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established alike, seven-transmembrane-spanning G protein-coupled receptors (GPCRs). More than 200 receptors that transduce many important physiological processes and are the target for about 50% of all drugs have been identified in this family (.–.) . A further approx 160 or so ″orphan″ GPCRs have been predicteDiskectomy 发表于 2025-3-26 18:36:15
Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels,s have been studied via selective inhibitors, ion channels using naturally occurring toxins, and second messenger systems using inositol polyphosphates. Ligand binding complements immunohistochemistry (..) and . hybridization (..) by permitting pharmacological characterization and quantification of