Paradox 发表于 2025-3-26 21:44:13

Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts,ty in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or conditions that might change observed in vitro specificity.

Cumbersome 发表于 2025-3-27 02:26:34

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重力 发表于 2025-3-27 06:31:04

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微粒 发表于 2025-3-27 13:06:29

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JEER 发表于 2025-3-27 17:31:16

Analysis of the Interactions Between Rab GTPases and Class V Myosins,and organelles such as the endoplasmic reticulum. The Rab GTPases play a critical role in recruiting class V myosins to their cargo. We recently published a study in which we used the yeast two-hybrid system to systematically test myosin Va for its ability to interact with each member of the human R

痛苦一下 发表于 2025-3-27 19:52:46

Ypt1 and TRAPP Interactions: Optimization of Multicolor Bimolecular Fluorescence Complementation inllular sites of these interactions, we have employed a number of approaches. One approach that we have recently optimized for the use in yeast is multicolor bimolecular fluorescence complementation (BiFC). BiFC, which employs split fluorescent tags, has emerged as a powerful approach for determining

PAEAN 发表于 2025-3-28 01:16:39

Functional Analysis of Rab27A and Its Effector Slp2-a in Renal Epithelial Cells,es as the cells become polarized, and they are specifically localized at the apical membrane in polarized MDCK II cells (i.e., two-dimensional cell culture). Slp2-a is also localized at the apical membrane of tubular MDCK II cysts (i.e., three-dimensional cell culture) and promotes the formation of

事情 发表于 2025-3-28 05:53:29

Small GTPases in Acrosomal Exocytosis,s to determine the subcellular localization of active Rabs, something not achievable with other methods. By means of these techniques, we have reported that Rab27 and Rab3 act sequentially and are organized in a RabGEF cascade during the AR. Although we developed them to scrutinize the exocytosis of

意外的成功 发表于 2025-3-28 09:08:47

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NIB 发表于 2025-3-28 10:36:05

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查看完整版本: Titlebook: Rab GTPases; Methods and Protocol Guangpu Li Book 2015 Springer Science+Business Media New York 2015 Endocytic Pathways.Eukaryotes.Exocytic