新陈代谢
发表于 2025-3-28 17:35:33
1064-3745undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to ra
一夫一妻制
发表于 2025-3-28 22:24:34
Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in was provided by Wang et al. (.). As an internal standard, an RNA template with the same primer sites as the target mRNA was reverse-transcribed. The amplified PCR products differed in size and were separated by agarose gel electrophoresis. The presence of .P-labeled 5′ primer allowed quantification by scintillation counting.
DUCE
发表于 2025-3-29 00:39:03
Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytesding murine macrophages (.,.), human endothelial cells (.,.), eosinophils (.), and Leydig cells derived from human and mouse testis (.). We have demonstrated that human monocytes and lymphocytes express the SP gene mRNA transcripts and produce SP protein (.–.).
充气女
发表于 2025-3-29 03:41:20
Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCR-α/ribavirin combination therapies, patients with a high viral load respond more poorly to this treatment and require a longer duration of therapy. In addition, the HCV-RNA status in serum during treatment is used as a marker for the short-term success of therapy.
因无茶而冷淡
发表于 2025-3-29 09:04:44
Nested RT-PCRer amplicon (.). Because their sequences are different from the primary set of primers, they will not amplify artifacts or nonspecific products generated in the primary PCR, enabling product specificity to be maintained over the high number of amplification cycles combined in the primary and secondary PCRs.
Admire
发表于 2025-3-29 11:44:25
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完成才能战胜
发表于 2025-3-29 17:26:17
Joe O’ConnellIncludes supplementary material:
Water-Brash
发表于 2025-3-29 22:17:25
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炼油厂
发表于 2025-3-30 01:50:06
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Canyon
发表于 2025-3-30 06:59:04
om-Spannungsbeziehungen für die Netzwerkelemente bestimmt. Im folgenden sollen systematische Verfahren entwickelt werden, die dazu geeignet sind, auch kompliziertere Netzwerke in verhältnismäßig einfacher Weise zu analysieren und allgemeine Aussagen zu machen. Unter einem Netzwerk wird im Sinne von