COUCH
发表于 2025-3-30 09:10:27
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Overdose
发表于 2025-3-30 12:36:05
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jovial
发表于 2025-3-30 17:53:56
Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts,to regulate gene expression through RNAi they must be loaded, or “programmed,” with a single strand of small RNA. Natural small RNAs are typically double-stranded duplexes that require additional factors for efficient and specific loading into Ago proteins. Here, a protocol is described for investig
Ptsd429
发表于 2025-3-30 22:42:49
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Phenothiazines
发表于 2025-3-31 01:04:34
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fastness
发表于 2025-3-31 05:41:13
Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles,. In eukaryotes and archaea, RNA pseudouridylation is catalyzed largely by box H/ACA ribonucleoproteins (RNPs), a distinct group of RNA–protein complexes each consisting of a unique RNA and four common proteins. The RNA component of the complex serves as a guide that base-pairs with its substrate RN
CAMEO
发表于 2025-3-31 10:02:57
,Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixtu to prepare .P-labeled RNA probes and to use them to assay for RNA–protein interactions after partially purified protein preparations are resolved on denaturing SDS-polyacrylamide gels. The method can unambiguously determine whether the protein of interest can directly and independently bind RNA eve
伪造者
发表于 2025-3-31 15:13:39
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失望未来
发表于 2025-3-31 21:07:12
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内部
发表于 2025-4-1 01:03:23
Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichmentation of the complete set of mRNA-binding proteins is a key step in understanding the regulation of gene expression. Herein, we describe a method for identifying yeast mRNA-binding proteins in a systematic manner using UV crosslinking, purification of polyA(+) mRNAs under denaturing conditions, and