驳船
发表于 2025-3-28 17:23:50
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碎石头
发表于 2025-3-28 20:37:55
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协迫
发表于 2025-3-29 00:38:57
Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging,ly used for the cellular imaging, but the choice of targets is still limited. Recently, fluorogenic RNA-based sensors gained lots of attention. This novel sensor system can function as a general platform for various cellular targets. Here, we describe the steps to rationally design, optimize, and ap
星球的光亮度
发表于 2025-3-29 05:57:36
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枫树
发表于 2025-3-29 09:18:34
,FRET Analysis of RNA –Protein Interactions Using Spinach Aptamers,ery of fluorescent light-up aptamers (FLAPs), short RNA aptamers that switch the fluorescence of small, cell-permeable, and nontoxic organic chromophores on, paved the road for their utilization in direct RNA–protein interactions. The combination with fluorescent proteins as biological fluorophores
斗志
发表于 2025-3-29 15:15:22
Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vi aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofa
detach
发表于 2025-3-29 15:51:12
Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells,itch libraries are constructed by randomizing the key nucleotides that couple the molecular recognition function of an aptamer with the self-cleavage activity of a ribozyme. The allosteric ribozyme (aptazyme) candidates are cloned in the 3′ untranslated region (UTR) of a reporter gene mRNA. The plas
inclusive
发表于 2025-3-29 23:37:56
,Folding RNA–Protein Complex into Designed Nanostructures,otifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nanoobjects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we desc
Lymphocyte
发表于 2025-3-29 23:57:58
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Biguanides
发表于 2025-3-30 05:02:46
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