Heretical
发表于 2025-3-26 21:42:51
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乞丐
发表于 2025-3-27 03:16:54
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无所不知
发表于 2025-3-27 07:17:34
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腐败
发表于 2025-3-27 09:33:21
Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryafish embryos, an smFISH protocol optimized for zebrafish cryosections, and a user-friendly, automated analysis pipeline for cell segmentation and transcript detection. The software is freely available and can be used to analyze sections of any multicellular organism.
机制
发表于 2025-3-27 14:33:48
In Situ Detection of MicroRNA Expression with RNAscope Probes, provides simultaneous single molecule detection of individual microRNA and its target gene. This method allows for rapid and sensitive detection of noncoding RNA transcripts in frozen tissue sections.
愤慨一下
发表于 2025-3-27 20:46:59
Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH, entangled experimental results. Using this method, we observe in living brain tissue spatially constrained nuclear RNA foci under dynamic regulation in response to cellular transcriptional activity with individual cell heterogeneity.
胆大
发表于 2025-3-27 23:33:11
Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers,orophores and also dual-color imaging of two distinct RNAs in live bacteria. Our approach uses small RNA tags and small molecule fluorophores for labeling, thereby minimal perturbation on the function and dynamics of the RNA of interest is expected.
AMOR
发表于 2025-3-28 02:40:19
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Neonatal
发表于 2025-3-28 07:23:02
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wangle
发表于 2025-3-28 14:24:37
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