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Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells,membranes before extraction. Here, I describe preparation of Triton X-100-insoluble DRMs from cultured mammalian cells on sucrose gradients. Methods for analyzing the distribution of particular proteins across the gradient, and for recovering DRMs for further use are presented.
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查看完整版本: Titlebook: Power and Conflict in the Age of Transparency; Bernard I. Finel,Kristin M. Lord Book 2000 Bernard I. Finel and Kristin M. Lord 2000 confli