Infuriate
发表于 2025-3-26 21:46:39
High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing,hatic amine or hydroxide anion). We describe design, synthesis, and purification of fimers, and their use in protocols for direct sequencing of genomic deoxyribonucleic acid (DNA). Protocol for isolation of microbial genomic DNA is also reported.
减震
发表于 2025-3-27 04:28:11
Nonenzymatic Template-Directed RNA Synthesis, chromatography or on gel electrophoresis with on-line detection applying a deoxyribonucleic acid sequencer. Three examples of primer extensions are given, one of which demonstrates how the reaction of several primers may be monitored simultaneously.
GIST
发表于 2025-3-27 07:25:55
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Inflamed
发表于 2025-3-27 12:51:31
Locked Nucleic Acid Synthesis,oved from the solid support as the 5′-end-.dimethoxytrityl (DMT) protected LNA oligomer by treatment with concentrated aqueous ammonia that also removes the phosphate and nucleobase protecting groups. The crude DMT protected LNA product can be purified using, for example, reversed-phase chromatography.
committed
发表于 2025-3-27 13:50:58
Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides, inhibitor camptothecin to a triple helix-forming oligonucleotide is taken as an example of such synthesis. The described method has general interest because any functional ligand containing a primary or secondary amino group or aliphatic carboxyl group could be attached to the terminal phosphate of an oligonucleotide in a similar way.
Ligament
发表于 2025-3-27 21:27:38
Methods in Molecular Biologyhttp://image.papertrans.cn/o/image/700862.jpg
CAB
发表于 2025-3-27 23:52:02
https://doi.org/10.1385/1592598234DNA; PCR; gene expression; genes; siRNA
attenuate
发表于 2025-3-28 05:58:30
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Irascible
发表于 2025-3-28 07:25:57
Oligonucleotide Synthesis978-1-59259-823-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
OREX
发表于 2025-3-28 11:57:29
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