植物学
发表于 2025-3-23 12:27:50
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种族被根除
发表于 2025-3-23 17:47:27
Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.
能量守恒
发表于 2025-3-23 21:59:58
Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopynd tissue samples that can be re-probed whenever new antibodies or probes become available. Rapid and robust protocols are available for both I-EM and EM-ISH post-embedding techniques so that they can be combined on the same sections, providing ultrastructural and molecular insights into newly “emerging” nuclear bodies.
podiatrist
发表于 2025-3-23 22:32:41
Cross-Linking and Immunoprecipitation of Nuclear RNA-Binding Proteinsution. Here, we describe in detail a PAR-CLIP protocol to prepare cDNA libraries for high-throughput sequencing from RNA fragments that are bound to RBPs not only in the nucleus but also in the cytoplasm.
价值在贬值
发表于 2025-3-24 05:45:06
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煞费苦心
发表于 2025-3-24 07:12:52
https://doi.org/10.1007/978-1-4939-2253-6biochemical; cytological; eukaryotes; gene expression; long non-coding RNAs; molecular biological methods
averse
发表于 2025-3-24 12:05:24
978-1-4939-5549-7Springer Science+Business Media New York 2015
极少
发表于 2025-3-24 18:22:39
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钻孔
发表于 2025-3-24 21:09:54
Shinichi Nakagawa,Tetsuro HiroseIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
允许
发表于 2025-3-25 01:40:28
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