大炮 发表于 2025-3-28 17:20:21
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https://doi.org/10.1007/3-540-45463-2Algebra; Electronics; Noise; Oscillators; Phase; calculus; chaos; communication; geometry; metrology; number t高贵领导 发表于 2025-3-29 05:35:46
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Pierre Cartieronstruction process. The application of this toolkit was successfully demonstrated by perturbation of genomes of . A3(2) and . Tü 365. The CRISPR-Cas9 toolkit and related protocol described here can be widely used for metabolic engineering of actinomycetes.cinder 发表于 2025-3-29 22:57:37
Courty Jean-Michel,Francesca Grassia,Serge Reynaudntegration of the carotenoid pathway from 15 exogenously supplied DNA parts into three targeted genomic loci. As a second proof-of-principle, a total of ten DNA parts were assembled and integrated in two genomic loci to construct a tyrosine production strain, and at the same time knocking out two ge大方一点 发表于 2025-3-30 00:06:15
V. Pasquiers are added to the ends of the BAC vector. Second, two restriction enzyme sites are introduced into the respective sides of the designed overlaps to achieve the hierarchical assembly of large DNA molecules. The optimized Gibson assembly method facilitates fast acquisition of large DNA fragments with勉强 发表于 2025-3-30 04:29:13
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