船员 发表于 2025-3-23 12:01:18
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David S. Wilcover documents the most commonly used procedures for making synaptosomes, indicates the most likely contaminants present after each procedure, and assesses the viability of the resulting synaptosomes. This provides researchers with a decision tool to determine which synaptosome preparation procedure beIndicative 发表于 2025-3-24 02:07:52
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David S. Wilcovehich are of neuronal origin. The procedure produces mitochondria that (1) have minimal cytoplasmic contamination and (2) are functionally active based on measurements of mitochondrial matrix protein import; the process depends on the mitochondrial integrity and presence of high membrane potential. TLINE 发表于 2025-3-24 09:58:48
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David S. Wilcoveaptosomes from whole mammalian brain tissue, at two levels of purity: the “crude” P2 and the Dunkley S1 method of Percoll-purified fractions, for molecular, biochemical, and functional assays. These represent the shortest experimental methods to date. The protocol involves mild homogenization of the间接 发表于 2025-3-24 15:09:46
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David S. WilcoveReveals a little-known side effect of climate change and environmental degradation: the decline of migration.Great animal migrations provide important, tangible benefits to humanity.Wilcove’s passion