Preserve
发表于 2025-3-28 15:50:30
David C. Hondius,Jeroen J. M. Hoozemans,Annemieke J. M. Rozemuller,August B. Smit that 60 % of noninfectious diseases are caused by errors in splicing, making the study of pre-mRNA splicing a high priority from a health perspective. Pre-mRNA splicing is also complicated: the molecular machine that catalyzes the reaction, the spliceosome, is composed of five small nuclear RNAs, a
NICE
发表于 2025-3-28 22:27:58
http://reply.papertrans.cn/67/6644/664384/664384_42.png
预防注射
发表于 2025-3-29 00:50:05
http://reply.papertrans.cn/67/6644/664384/664384_43.png
Astigmatism
发表于 2025-3-29 04:24:30
Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue, the fresh brain provides clear advantages, such as being able to see structures in very great detail by differences in color and shade, it also has some disadvantages. This particularly becomes evident when processing multiple samples within a short time-window, e.g., after a physiological stimulus
滑动
发表于 2025-3-29 09:45:39
Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centriiton X-100 and sucrose density centrifugation. The protocol also includes purification of other synapse sub-domains such as synaptosome, synaptic plasma membrane (SPM), synaptic membrane raft, PSD lattice, . (nuclei and cell debris), . (crude mitochondria fraction), . (soluble fraction), and . (micr
赏心悦目
发表于 2025-3-29 13:53:18
http://reply.papertrans.cn/67/6644/664384/664384_46.png
Haphazard
发表于 2025-3-29 18:37:41
High-pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics,H reversed-phase fractionation with low-pH LC-MS analysis is the method of choice, because it provides the optimal separation efficiency and deep protein coverage. In this chapter, we illustrate the protocols of HPLC-based and the tip-based high-pH reversed-phase chromatographic fractionation of pep
GROWL
发表于 2025-3-29 23:24:55
http://reply.papertrans.cn/67/6644/664384/664384_48.png
Frisky
发表于 2025-3-30 00:11:26
Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip,d biomolecules by using a phosphate-binding zinc(II) complex of 1,3-bis(pyridin-2-ylmethylamino)propan-2-olate (Phos-tag). A 200-μL micropipette tip containing 10 μL of swollen agarose beads functionalized with Phos-tag moieties (Phos-tag Tip) was prepared. All steps in the phosphate-affinity separa
公社
发表于 2025-3-30 04:41:50
,Integrated Immunoprecipitation: Blue Native Gel Electrophoresis—Mass Spectrometry for the Identific and cellular state. To understand their biology, there is a need to identify the constituents in these various protein complexes. Conventional immunoprecipitation combined with proteomics analysis is nowadays routinely used to identify multiple interactors of a bait protein; however, it is unable t